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脂肪组织存在菌群再添新证,与肥胖和糖尿病的炎症相关

创作:Vicky审核:何才高2020-08-06

①纳入75名肥胖者,部分患2型糖尿病(T2D),在血液以及多种脂肪组织(AT)中鉴定出细菌DNA,浓度范围为0.1-5 pg/μg,并用荧光原位杂交方法确定是活菌;

②变形菌和厚壁菌是AT中的优势细菌,肠系膜AT是细菌移位的主要部位,其细菌多样性在几个组织中最高;

③AT细菌数量与免疫细胞浸润、炎症和代谢指标相关;

④T2D和非T2D者的组织菌群组成不同,与全身和组织特异性炎症标志物等临床指标相关;

⑤细菌DNA可刺激脂肪细胞在体外表达TNFα和IL6。


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主编推荐语

动物研究显示,“肠漏”及其相关的菌群移位,可能是促进代谢疾病的一种机制。最近在Nature Metabolism上发表的一项研究也显示,肥胖患者的不同脂肪组织中存在细菌DNA。《Gut》发表的一项最新研究,经严格的实验设计和生物信息学处理以排除污染干扰,进一步提供了脂肪组织中存在菌群的证据。研究者认为,这些菌群可能主要源自肠道,在触发和维持脂肪组织亚临床炎症中有重要作用,影响肥胖者的代谢健康。

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本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
Gut
[IF:19.819]
Adipose tissue derived bacteria are associated with inflammation in obesity and type 2 diabetes
源于脂肪组织的细菌与肥胖和2型糖尿病的炎症相关
10.1136/gutjnl-2019-320118
04-21, Article
Abstract:
Objective: Bacterial translocation to various organs including human adipose tissue (AT) due to increased intestinal permeability remains poorly understood. We hypothesised that: (1) bacterial presence is highly tissue specific and (2) related in composition and quantity to immune inflammatory and metabolic burden.
Design : We quantified and sequenced the bacterial 16S rRNA gene in blood and AT samples (omental, mesenteric and subcutaneous) of 75 subjects with obesity with or without type 2 diabetes (T2D) and used catalysed reporter deposition (CARD) – fluorescence in situ hybridisation (FISH) to detect bacteria in AT.
Results : Under stringent experimental and bioinformatic control for contaminants, bacterial DNA was detected in blood and omental, subcutaneous and mesenteric AT samples in the range of 0.1 to 5 pg/µg DNA isolate. Moreover, CARD-FISH allowed the detection of living, AT-borne bacteria. Proteobacteria and Firmicutes were the predominant phyla, and bacterial quantity was associated with immune cell infiltration, inflammatory and metabolic parameters in a tissue-specific manner. Bacterial composition differed between subjects with and without T2D and was associated with related clinical measures, including systemic and tissues-specific inflammatory markers. Finally, treatment of adipocytes with bacterial DNA in vitro stimulated the expression of TNFA and IL6.
Conclusions: Our study provides contaminant aware evidence for the presence of bacteria and bacterial DNA in several ATs in obesity and T2D and suggests an important role of bacteria in initiating and sustaining local AT subclinical inflammation and therefore impacting metabolic sequelae of obesity.